Protocol Collection

The following protocols are available:

• Protocols for cell-free systems
• Apoptosis Assays
• Cell lysis protocols
• Cell culture protocols
• Transfection protocols

Protocols for cell-free systems

• Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
• Preparation of cytoplasmic extracts for the application in a cell-free system (apoptosis assay)
• Isolation of cell nuclei for the application in a cell-free system (apoptosis assay)

Apoptosis assays

• Radioactive DNA Fragmentation Assay
• Radioactive Proliferation Assay
• DNA laddering assay for treated cells
• DAPI staining of cells

Cell lysis protocols

• Gentle lysis of mammalian cells for cytochrome c release assay

Cell culture protocols

• Culture Conditions for Human Prostatic Carcinoma Cell lines PC3, DU145, JCA1, ALVA31, and LNCaP

Gel shift assays

• EMSA for the detection of nuclear NF-kB